Characterization of human DNA polymerase delta and its immunochemical relationships with DNA polymerase alpha and epsilon

Characterization of Human DNA Polymerase 6 and Its Immunochemical Relationships with DNA Polymerase ( I ! and

DNA polymerase 6 was purified from human placenta and its polymerase catalytic subunit identified as a 125-kDa polypeptide by activity staining. This 125kDa form of DNA polymerase 6 resembles that reported from calf thymus (Lee, M. Y. W. T., Tan, C.-K., Downey, K. M., and So, A. G . (1984) Biochemistry 23, 1906-1913) and differs in molecular properties from a previously described form isolated ...

Characterization of Human DNA Polymerase Delta and Its Subassemblies Reconstituted by Expression in the Multibac System

Mammalian DNA polymerase δ (Pol δ), a four-subunit enzyme, plays a crucial and versatile role in DNA replication and DNA repair processes. We have reconstituted human Pol δ complexes in insect cells infected with a single baculovirus into which one or more subunits were assembled. This system allowed for the efficient expression of the tetrameric Pol δ holoenzyme, the p125/p50 core dimer, the c...

DNA repair synthesis in human fibroblasts requires DNA polymerase delta.

When UV-irradiated cultured diploid human fibroblasts were permeabilized with Brij-58 then separated from soluble material by centrifugation, conservative DNA repair synthesis could be restored by a soluble factor obtained from the supernatant of similarly treated HeLa cells. Extensive purification of this factor yielded a 10.2 S, 220,000-dalton polypeptide with the DNA polymerase and 3'- to 5'...

Further Characterization of Human DNA Polymerase

Polymerase interacting protein 38 (PDIP38) was identified as a human DNA polymerase (pol) interacting protein through a direct interaction with p50, the small subunit of human pol . PDIP38 was also found to interact with proliferating cell nuclear antigen, which suggested that it might play a role in vivo in the processes of DNA replication and DNA repair in the nucleus. In order to characteriz...

Role of yeast DNA polymerase epsilon during DNA replication